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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
02/09/2015 |
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Data da última atualização: |
17/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CATARINO, A. de M.; FAJARDO, T. V. M.; PIO-RIBEIRO, G.; EIRAS, M.; NICKEL, O. |
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Afiliação: |
THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV. |
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Título: |
Incidência de vírus em videiras no Nordeste brasileiro e caracterização molecular parcial de isolados virais locais. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Ciência Rural: Santa Maria, v. 45, n. 3, p. 379-385, mar. 2015. |
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ISSN: |
0103-8478 |
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DOI: |
10.1590/0103-8478cr20140587 |
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Idioma: |
Português |
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Conteúdo: |
Os objetivos deste trabalho foram identificar as espécies virais presentes em vinhedos comerciais de duas regiões do Nordeste do Brasil e realizar a caracterização molecular parcial de isolados de três espécies virais. A diagnose foi realizada por meio de RT-PCR em tempo real para a detecção de Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine leafroll-associated virus 2, 3 e 4 (GLRaV-2, -3 e -4), Grapevine fleck virus (GFkV), Grapevine rupestris vein feathering virus (GRVFV) e Grapevine fanleaf virus (GFLV). Exceto para GFLV, os vírus avaliados estão amplamente disseminados nas áreas amostradas, frequentemente em altas incidências e em infecções múltiplas, de até 98% e 76,4%, na Zona da Mata e no Vale do São Francisco, respectivamente. Isolados locais de GVA, GVB e GLRaV-3 foram parcialmente caracterizados com base na sequência completa de nucleotídeos do gene da proteína capsidial e apresentaram alta porcentagem de identidade de nucleotídeos com outros isolados brasileiros: 91,2% (GVA), 99,8% (GVB) e 99,7% (GLRaV-3). Palavras-chave: proteína capsidial, levantamento, detecção, variabilidade, Vitis. |
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Palavras-Chave: |
Coat protein; Espécies virais; Proteína capsidial; Variabilidade; Videira. |
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Thesagro: |
Levantamento; Uva. |
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Thesaurus Nal: |
Vitis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/128988/1/THOR-CienciaRual-v45-n3-p379-385-2015.pdf
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Marc: |
LEADER 02063naa a2200289 a 4500
001 2023196
005 2016-03-17
008 2015 bl uuuu u00u1 u #d
022 $a0103-8478
024 7 $a10.1590/0103-8478cr20140587$2DOI
100 1 $aCATARINO, A. de M.
245 $aIncidência de vírus em videiras no Nordeste brasileiro e caracterização molecular parcial de isolados virais locais.$h[electronic resource]
260 $c2015
520 $aOs objetivos deste trabalho foram identificar as espécies virais presentes em vinhedos comerciais de duas regiões do Nordeste do Brasil e realizar a caracterização molecular parcial de isolados de três espécies virais. A diagnose foi realizada por meio de RT-PCR em tempo real para a detecção de Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine leafroll-associated virus 2, 3 e 4 (GLRaV-2, -3 e -4), Grapevine fleck virus (GFkV), Grapevine rupestris vein feathering virus (GRVFV) e Grapevine fanleaf virus (GFLV). Exceto para GFLV, os vírus avaliados estão amplamente disseminados nas áreas amostradas, frequentemente em altas incidências e em infecções múltiplas, de até 98% e 76,4%, na Zona da Mata e no Vale do São Francisco, respectivamente. Isolados locais de GVA, GVB e GLRaV-3 foram parcialmente caracterizados com base na sequência completa de nucleotídeos do gene da proteína capsidial e apresentaram alta porcentagem de identidade de nucleotídeos com outros isolados brasileiros: 91,2% (GVA), 99,8% (GVB) e 99,7% (GLRaV-3). Palavras-chave: proteína capsidial, levantamento, detecção, variabilidade, Vitis.
650 $aVitis
650 $aLevantamento
650 $aUva
653 $aCoat protein
653 $aEspécies virais
653 $aProteína capsidial
653 $aVariabilidade
653 $aVideira
700 1 $aFAJARDO, T. V. M.
700 1 $aPIO-RIBEIRO, G.
700 1 $aEIRAS, M.
700 1 $aNICKEL, O.
773 $tCiência Rural: Santa Maria$gv. 45, n. 3, p. 379-385, mar. 2015.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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Data corrente: |
26/05/2017 |
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Data da última atualização: |
21/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BUZANSKAS, M. E.; VENTURA, R. V.; CHUD, T. C. S.; BERNARDES, P. A.; SANTOS, D. J. de A.; REGITANO, L. C. de A.; ALENCAR, M. M. de; MUDADU, M. de A.; ZANELLA, R.; SILVA, M. V. G. B.; LI, C.; SCHENKE, F. S.; MUNARI, D. P. |
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Afiliação: |
Marcos Eli Buzanskas, Unesp; Ricardo Vieira Ventura, USP; Tatiane Cristina Seleguim Chud, Unesp; Priscila Arrigucci Bernardes, Unesp; Daniel Jordan de Abreu Santos, Unesp; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; MAURICIO MELLO DE ALENCAR, CPPSE; MAURICIO DE ALVARENGA MUDADU, CNPTIA; Ricardo Zanella, UPF; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; Changxi Li, University of Alberta; Flavio Schramm Schenke, University of Guelph; Danísio Prado Munari, Unesp. |
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Título: |
Study on the introgression of beef breeds in canchim cattle using single nucleotide polymorphism markers. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plos one, v. 12, p. 1-16, 2017. |
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DOI: |
https://doi.org/10.1371/journal.pone.0171660 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the level of introgression of breeds in the Canchim (CA: 62.5% Charolais?37.5% Zebu) and MA genetic group (MA: 65.6% Charolais?34.4% Zebu) cattle using genomic information on Charolais (CH), Nelore (NE), and Indubrasil (IB) breeds. The number of animals used was 395 (CA and MA), 763 (NE), 338 (CH), and 37 (IB). The Bovine50SNP BeadChip from Illumina panel was used to estimate the levels of introgression of breeds considering the Maximum likelihood, Bayesian, and Single Regression method. After genotype quality control, 32,308 SNPs were considered in the analysis. Furthermore, three thresholds to prune out SNPs in linkage disequilibrium higher than 0.10, 0.05, and 0.01 were considered, resulting in 15,286, 7,652, and 1,582 SNPs, respectively. For k = 2, the proportion of taurine and indicine varied from the expected proportion based on pedigree for all methods studied. For k = 3, the Regression method was able to differentiate the animals in three main clusters assigned to each purebred breed, showing more reasonable according to its biological viewpoint. Analyzing the data considering k = 2 seems to be more appropriate for Canchim-MA animals due to its biological interpretation. The usage of 32,308 SNPs in the analyses resulted in similar findings between the estimated and expected breed proportions. Using the Regression approach, a contribution of Indubrasil was observed in Canchim-MA when k = 3 was considered. Genetic parameter estimation could account for this breed composition information as a source of variation in order to improve the accuracy of genetic models. Our findings may help assemble appropriate reference populations for genomic prediction for Canchim-MA in order to improve prediction accuracy. Using the information on the level of introgression in each individual could also be useful in breeding or crossing design to improve individual heterosis in crossbred cattle. MenosThe aim of this study was to evaluate the level of introgression of breeds in the Canchim (CA: 62.5% Charolais?37.5% Zebu) and MA genetic group (MA: 65.6% Charolais?34.4% Zebu) cattle using genomic information on Charolais (CH), Nelore (NE), and Indubrasil (IB) breeds. The number of animals used was 395 (CA and MA), 763 (NE), 338 (CH), and 37 (IB). The Bovine50SNP BeadChip from Illumina panel was used to estimate the levels of introgression of breeds considering the Maximum likelihood, Bayesian, and Single Regression method. After genotype quality control, 32,308 SNPs were considered in the analysis. Furthermore, three thresholds to prune out SNPs in linkage disequilibrium higher than 0.10, 0.05, and 0.01 were considered, resulting in 15,286, 7,652, and 1,582 SNPs, respectively. For k = 2, the proportion of taurine and indicine varied from the expected proportion based on pedigree for all methods studied. For k = 3, the Regression method was able to differentiate the animals in three main clusters assigned to each purebred breed, showing more reasonable according to its biological viewpoint. Analyzing the data considering k = 2 seems to be more appropriate for Canchim-MA animals due to its biological interpretation. The usage of 32,308 SNPs in the analyses resulted in similar findings between the estimated and expected breed proportions. Using the Regression approach, a contribution of Indubrasil was observed in Canchim-MA when k = 3 was considered. Genetic parameter estimat... Mostrar Tudo |
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Palavras-Chave: |
Canchim cattle; Polimorfismo de nucleotídeo único; Raças de gado. |
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Thesagro: |
Gado canchim; Gado de corte; Gado Indubrasil; Gado nelore. |
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Thesaurus NAL: |
beef cattle; Cattle breeds; Charolais; Composite breeds; polymorphism; Single nucleotide polymorphism. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160251/1/journal.pone.0171660.pdf
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Marc: |
LEADER 03213naa a2200433 a 4500
001 2070093
005 2019-05-21
008 2017 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0171660$2DOI
100 1 $aBUZANSKAS, M. E.
245 $aStudy on the introgression of beef breeds in canchim cattle using single nucleotide polymorphism markers.$h[electronic resource]
260 $c2017
520 $aThe aim of this study was to evaluate the level of introgression of breeds in the Canchim (CA: 62.5% Charolais?37.5% Zebu) and MA genetic group (MA: 65.6% Charolais?34.4% Zebu) cattle using genomic information on Charolais (CH), Nelore (NE), and Indubrasil (IB) breeds. The number of animals used was 395 (CA and MA), 763 (NE), 338 (CH), and 37 (IB). The Bovine50SNP BeadChip from Illumina panel was used to estimate the levels of introgression of breeds considering the Maximum likelihood, Bayesian, and Single Regression method. After genotype quality control, 32,308 SNPs were considered in the analysis. Furthermore, three thresholds to prune out SNPs in linkage disequilibrium higher than 0.10, 0.05, and 0.01 were considered, resulting in 15,286, 7,652, and 1,582 SNPs, respectively. For k = 2, the proportion of taurine and indicine varied from the expected proportion based on pedigree for all methods studied. For k = 3, the Regression method was able to differentiate the animals in three main clusters assigned to each purebred breed, showing more reasonable according to its biological viewpoint. Analyzing the data considering k = 2 seems to be more appropriate for Canchim-MA animals due to its biological interpretation. The usage of 32,308 SNPs in the analyses resulted in similar findings between the estimated and expected breed proportions. Using the Regression approach, a contribution of Indubrasil was observed in Canchim-MA when k = 3 was considered. Genetic parameter estimation could account for this breed composition information as a source of variation in order to improve the accuracy of genetic models. Our findings may help assemble appropriate reference populations for genomic prediction for Canchim-MA in order to improve prediction accuracy. Using the information on the level of introgression in each individual could also be useful in breeding or crossing design to improve individual heterosis in crossbred cattle.
650 $abeef cattle
650 $aCattle breeds
650 $aCharolais
650 $aComposite breeds
650 $apolymorphism
650 $aSingle nucleotide polymorphism
650 $aGado canchim
650 $aGado de corte
650 $aGado Indubrasil
650 $aGado nelore
653 $aCanchim cattle
653 $aPolimorfismo de nucleotídeo único
653 $aRaças de gado
700 1 $aVENTURA, R. V.
700 1 $aCHUD, T. C. S.
700 1 $aBERNARDES, P. A.
700 1 $aSANTOS, D. J. de A.
700 1 $aREGITANO, L. C. de A.
700 1 $aALENCAR, M. M. de
700 1 $aMUDADU, M. de A.
700 1 $aZANELLA, R.
700 1 $aSILVA, M. V. G. B.
700 1 $aLI, C.
700 1 $aSCHENKE, F. S.
700 1 $aMUNARI, D. P.
773 $tPlos one$gv. 12, p. 1-16, 2017.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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